《植物生理学报》 2009, 45(9): 859-864

小麦成熟胚脱分化过程中WRKY 家族转录因子基因及其下游基因的表达

崔琰, 舒文涛, 赵一丹, 陈新建, 陈军营*

河南农业大学农学院, 郑州450002

通信作者：陈军营；E-mail: chenjunying3978@126.com；Tel: 0371-63558126

摘　要：

用Affymetrix小麦基因芯片研究了小麦成熟胚在MS+2,4-D (2 mg•L-1)培养基上脱分化过程中不同时间点的基因表达 变化, 用 NCBI、DATF 和 DRTF 等生物信息学相关网站对基因表达信息进行处理, 并针对 WRKY 家族转录因子相关基因 的变化情况进行分析的结果表明: WRKY家族中有20个相关基因在脱分化过程中的不同时点至少发生一次上调或下调表 达变化, 变化幅度分别为2~40倍和2~16倍, 其中WRKY6、WRKY18、WRKY33及其下游基因CA692019、CA660172、CA640435 等与小麦成熟胚脱分化过程可能有密切关系。WRKY6在脱分化的全过程中一直呈下降趋势, 推测2,4-D诱导可能导致成熟 胚细胞的抗性下降, 从而有利于脱分化的进行。WRKY33基因可能在脱分化初期的信号转导中有作用。不同物种的组织或 器官脱分化过程中的转录因子基因表达有差异, 说明其脱分化机制的多样性和复杂性。

关键词：小麦成熟胚; 脱分化; 基因芯片; WRKY家族转录因子基因

收稿：2009-06-16   修定：2009-07-21

资助：国家转基因植物研究与产业化专项基金(JY03-B-19-2)和河南省杰出人才创新基金(022100090)。

Expressions of Transcription Factor Genes in WRKY Family and Their Target Genes during Dedifferentiation of Mature Wheat (Triticum aestivum L.) Embryos

CUI Yan, SHU Wen-Tao, ZHAO Yi-Dan, CHEN Xin-Jian, CHEN Jun-Ying*

College of Agronomy, Henan Agricultural University, Zhengzhou 450002, China

Corresponding author: CHEN Jun-Ying; E-mail: chenjunying3978@126.com; Tel: 0371-63558126

Abstract:

The expression profiles of transcription factor genes in WRKY family and their target genes were analyzed using Affymetrix wheat GeneChip in this study during dedifferentiation of mature wheat (Triticum aestivum) embryos which cultured on MS medium supplemented with 2,4-D (2 mg·L^-1) at different time points of 2, 6, 12, 24 and 72 h. The information was processed using bioinformatics tools at NCBI, DATF, and DRTF websites. The result showed that there were 20 transcription factor genes in WRKY family and their target genes changed significantly in up- or down-regulation ranged 2–40 or 2–16 times at least one time point in mature wheat embryos dedifferentiation. According to the known functions of WRKY transcription factors and their changed trend, it was predicted that these genes, especially, WRKY6, WRKY18 and WRKY33 and their target genes CA692019, CA660172 and CA640435, might play roles in dedifferentiation of mature wheat embryos. WRKY6 was down-regulation in dedifferentiation and this indicted that 2,4-D might induce the low tolerance of mature wheat embryos to the stress of environment and thus facilitate the initial of dedifferentiation and its proceeding. WRKY33 might play a role in signal transduction in the early dedifferentiation stage. The different expressions of transcription factor genes in tissue and organ in different species implied the diversity and complex in dedifferentiation mechanism.

Key words: mature wheat embryos; dedifferentiation; gene microarray; WRKY transcription factor family